WASHINGTON (Reuters/AFP) - Al-Qaedas second-in-command on Friday accused the United States of leading a crusade to turn Pakistan into a divided nation and urged Pakistanis to join in a jihad to resist. The tape from Ayman al-Zawahri, the second this month, comes after Pakistani Taliban chief Baitullah Mehsud was killed in a US missile strike on Aug 5. The war in the tribal areas and Swat is an integral part of the crusade on Muslims across the world, Zawahri said in the tape posted on an Al-Qaeda-linked website. This is the battle, briefly and plainly; and this is why anyone who supports the Americans and Pakistan Army - under any pretext, ploy or lie - is in fact standing with, backing and supporting the Crusaders against Islam and Muslims, he said. He argued that the US military was using the Pakistani army as its proxy in the tribal areas and Swat as a means to defeat the insurgency in neighbouring Afghanistan. In the video address entitled Path of Doom he reiterates comments made in July calling for Pakistanis to wage war against the American crusaders and the Pakistani army. There is no honour for us except through Jihad, Zawahri said. People of Pakistan ... back the jihad and mujahideen with your persons, wealth, opinion, expertise, information and prayers and by exhorting others to help them and preach their message. The video, which SITE said was produced by Al-Qaedas media arm as-Sahab, took the form of a 22-minute, 30-second documentary with English subtitles. Zawahiri appeared with a bookshelf to his back as he has in previous videos.